INTERNATIONAL JOURNAL OF
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NTEGRATED MEDICAL
RESEARCH

pISSN: 2350-0360 eISSN: 2393-9869
 
 
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Pseudo thrombocytopenia: a true entity in automated hematological cell counter

Author: Vijay Suri1, Kuldeep Singh2, Gunjan Bala3*, Swati Yadav4, Saumya Bhagat5

1Professor, Department of Pathology, AIMSR, Bathinda

2Associate Professor, Department of Pathology, AIMSR, Bathinda

3Senior Resident, Department of Pathology, AIMSR, Bathinda

4Resident, Department of Pathology, AIMSR, Bathinda

5Assistant Professor, Department of Pathology, AIMSR, Bathinda

ABSTARCT

BACKGROUND: Thrombocytopenia is a condition in which platelet count is less than150,000 /µl. Pseudo thrombocytopenia is a spurious thrombocytopenia caused by anticoagulants dependent agglutinins. The aim of this study was to identify the cases in which spurious low platelet counts were given by the automated cell counter due to the anticoagulant EDTA. MATERIALS AND METHODS: This study was conducted in the Department of Pathology, AdeshInstitute of Medical Sciences & Research (AIMSR), Bathinda, Punjab from 1st April 2014 to 30th September 2015. A total of 16830 patients were investigated for hematology parameters including platelet counts, out of which 300 patients were flagged forthrombocytopenia using the automated hematology analyzer. All the blood samples were collected in EDTA as anticoagulant. These 300 subjects were finally enrolled in the study. All these 300 subjects were further evaluated by peripheral smear examination for assessing platelet counts. RESULTS: 27 subjects out of 300  of our study showed pseudo thrombocytopenia by automated analyzer with samples collected in EDTA ;Their platelet counts  rangedfrom 22× 109     µl to 148 × 109 µl While their peripheral smear examination  had platelet count ranged from 155 ×109   µl to 200 × 10µl by three different observers. CONCLUSION: Thrombocytopenia flagged in hematology analyzer should be screened on peripheral smear for platelets. PTCP is also labelled as a laboratory thrombocytopenia which can be induced by use of EDTA anticoagulant. Falsely low platelet count may lead to unnecessary, expensive and even invasive diagnostic or therapeutic procedure on the patients.

Keywords: pseudo thrombocytopenia, EDTA induced
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